|
KMID : 1161420200230020139
|
|
Journal of Medicinal Food
2020 Volume.23 No. 2 p.139 ~ p.146
|
|
|
Avocado/Soybean Unsaponifiables, Glucosamine and Chondroitin Sulfate Combination Inhibits Proinflammatory COX-2 Expression and Prostaglandin E2 Production in Tendon-Derived Cells
|
|
Grzanna Mark W.
Au Rebecca Y.
Au Angela Y.
Rashmir Ann M.
Frondoza Carmelita G.
|
|
|
Abstract
|
|
|
|
Tendinopathy, a common disorder in man and horses, is characterized by pain, dysfunction, and tendon degeneration. Inflammation plays a key role in the pathogenesis of tendinopathy. Tendon cells produce proinflammatory molecules that induce pain and tissue deterioration. Currently used nonsteroidal anti-inflammatory drugs are palliative but have been associated with adverse side effects prompting the search for safe, alternative compounds. This study determined whether tendon-derived cells' expression of proinflammatory cyclooxygenase (COX)-2 and production of prostaglandin E2 (PGE2) could be attenuated by the combination of avocado/soybean unsaponifiables (ASU), glucosamine (GLU), and chondroitin sulfate (CS). ASU, GLU, and CS have been used in the management of osteoarthritis-associated joint inflammation. Tenocytes in monolayer and microcarrier spinner cultures were incubated with media alone, or with the combination of ASU (8.3?μg/mL), GLU (11?μg/mL), and CS (20?μg/mL). Cultures were next incubated with media alone, or stimulated with interleukin-1β (IL-1β; 10?ng/mL) for 1?h to measure COX-2 gene expression, or for 24?h to measure PGE2 production, respectively. Tenocyte phenotype was analyzed by phase-contrast microscopy, immunocytochemistry, and Western blotting. Tendon-derived cells proliferated and produced extracellular matrix component type I collagen in monolayer and microcarrier spinner cultures. IL-1β-induced COX-2 gene expression and PGE2 production were significantly reduced by the combination of (ASU+GLU+CS). The suppression of IL-1β-induced inflammatory response suggests that (ASU+GLU+CS) may help attenuate deleterious inflammation in tendons.
|
|
|
KEYWORD
|
|
|
avocado/soybean unsaponifiables, chondroitin sulfate, COX-2, glucosamine, microcarrier spinner culture, PGE2, tendinopathy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|